s peptide tag peptide tag - S-Tag Antibody commonly used in molecular biology for protein purification and detection

S-tag protein The S peptide tag, a renowned 15-amino acid sequence derived from pancreatic ribonuclease A (RNase A), plays a crucial role in modern molecular biology. This small peptide, often referred to simply as S-tag, functions as an effective epitope tag, enabling researchers to easily detect, purify, and analyze recombinant proteins(PDF) S-peptide epitope tagging for protein purification, .... Its utility stems from a specific and robust interaction with its corresponding binding partner, ribonuclease S-protein, forming the basis of the S-Tag systemS•Tag : A Multipurpose Fusion Peptide for Recombinant ....

The S-peptide tag's origin from RNase A is significant. When RNase A is subjected to limited digestion, specifically by subtilisin, a single peptide bond is cleaved, yielding the S-peptide and the S-proteinS-Tag is an epitope tag composed of a 15 residue peptideand, together with S-protein, comprises pancreatic ribonuclease S (1).. The S-peptide itself is a key component that, when separated from the rest of the ribonuclease molecule, loses its enzymatic activity.S-tag is the name ofan oligopeptide derived from pancreatic ribonuclease A(RNase A). If RNase A is digested with subtilisin, a single peptide bond is cleaved. However, when recombined with the S-protein, it reconstitutes the active ribonuclease enzyme. This characteristic interaction is precisely what makes the S-tag so valuable as a tool for protein manipulation and studySynthetic peptide corresponding to S tag conjugated to keyhole limpet haemocyanin. Abbre, S-tag. Synonyms, S peptide epitope tag, KETAAAKFERQHMDS tag..

Applications of the S-Tag System

The primary applications of the S-tag revolve around its use as an epitope tag for recombinant proteins. Researchers commonly engineer the S-tag sequence onto either the N-terminus or C-terminus of a protein of interestS-Tag peptide. This fusion allows for a variety of downstream applications, including:

* Protein Purification: The strong affinity between the S-tag and S-protein can be leveraged in affinity chromatography. Immobilized S-protein on a solid support can capture tagged proteins from a complex mixture, enabling highly specific purification. This method is efficient and often results in proteins with high purityS-peptide Epitope Tag Antibody from Thermo Fisher ....

* Protein Detection and Visualization: Antibodies specifically designed to recognize the S-tag are readily available. These antibodies can be used in various immunological assays, such as Western blotting and enzyme-linked immunosorbent assays (ELISA), to detect the presence and quantity of the tagged protein.S Tag Peptide This is invaluable for confirming protein expression and monitoring protein levels in biological samples.

* Localization Studies: By fusing the S-tag to proteins whose cellular localization is being investigated, researchers can use anti-S-tag antibodies in techniques like immunofluorescence microscopyTheS-Tagis a novel fusionpeptide tagfor recombinant proteins that allows detection by a rapid, sensitive homogeneous assay or by colorimetric detection .... This allows for the visualization of where the tagged protein resides within the cell.

* Protein Interaction Studies: While not its primary function, the S-tag system can indirectly aid in studying protein interactions by facilitating the purification of specific protein complexes.

Advantages and Considerations

The S-tag offers several advantages that contribute to its widespread adoption. Its small size minimizes potential interference with the native function or structure of the target protein. The interaction with S-protein is highly specific, reducing the likelihood of non-specific binding and improving the accuracy of purification and detection. Furthermore, the S-Tag system is well-established, with a variety of reagents, including antibodies and purification kits, commercially available, making it accessible for many research laboratories.

However, as with any epitope tag, there are considerations. The target protein must be amenable to fusion with the S-tag without compromising its essential biological activity or folding.Epitope tagging is a powerful and versatile strategy for detecting and purifying proteinsexpressed by cloned genes. To utilize this feature, protein expression ... The presence of the tag might also influence protein solubility or stability in some cases. Additionally, while the S-peptide itself is derived from RNase A, the tagged protein will not possess RNase activity unless the S-protein is also present and the tag is correctly positioned for reconstitution.The S tag is a peptide sequence derived from ribonuclease Acommonly used in molecular biology for protein purification and detection. · Biological function ... Research has also explored the S-peptide's potential as a linker in protein cross-linking reactions, highlighting its versatility beyond simple taggingS-peptide Epitope Tag Monoclonal Antibody (6.2) (MA1-981).

In summary, the S peptide tag is a powerful and versatile tool in protein research, offering a reliable method for protein purification, detection, and analysis, underpinned by the specific molecular recognition between the S-tag peptide and ribonuclease S-protein.